Immobilization of glucoamylase on macroporous spheres
نویسندگان
چکیده
منابع مشابه
Immobilization of Glucoamylase on Macroporous Spheres
Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load, applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km val...
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Macroporous resin microbeads of methyl methacrylate-divinyl benzene copolymer were synthesized by radical suspension polymerization of acrolein with divinylbenzene in the presence of a pore-creating agent, petroleum ether. The microbeads had a large specific surface area and large pores covered the entire surface of the resin. This macroporous polymer carrier was aminated by hydrazine hydrate t...
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Porous nanospheres have a wide range of applications such as in catalysis, separation and controlled delivery. Among these nanospheres, syntheses and applications of porous silica nanospheres have been investigated extensively. Uniform porous silica nanospheres can be synthesized using a modified Stöber method. In the present study, porous silica spheres were prepared in the pre-formed emulsion...
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Folic acid (FA) was immobilized on Eu(3+)-doped nanoporous silica spheres (Eu:NPSs) through mediation of the 3-aminopropyltriethoxysilane adlayer. The ordered nanopores of Eu:NPS were preserved by the immobilization. The FA-immobilized Eu:NPSs showed the characteristic photoluminescence peak due to interactions between the FA molecules and Eu(3+) ions, and highly dispersed stability in phosphat...
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L. E. Shi, Z. X. Tang, Y. Yi, J. S. Chen, W. Y. Xiong, and G. Q. Ying College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, Zhejiang, China College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang, 310014, China Department of Food Science and Technology, College of Biotechnology, East China University of Science and Technology....
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ژورنال
عنوان ژورنال: Acta Periodica Technologica
سال: 2004
ISSN: 1450-7188,2406-095X
DOI: 10.2298/apt0435207m